PacBio and Covaris have introduced a joint workflow designed to enable robust HiFi sequencing of formalin-fixed, paraffin-embedded (FFPE) tissue samples, addressing long-standing challenges associated with DNA damage and fragmentation in archived specimens. Combining Covaris’ truXTRAC® FFPE extraction technology with PacBio’s Kinnex™ library preparation and Revio sequencing system, the integrated solution streamlines the process from sample preparation to sequencing while improving data quality and efficiency. The process takes advantage of Adaptive Focused Acoustics® technology to retrieve larger pieces of DNA and then join them together to achieve accurate sequencing results, thereby providing greater insights into the genome.
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In the verification experiments conducted on different types of cancers, the process was able to produce more than 100 million HiFi reads per sample, outperforming short-read sequencing in terms of identifying structural variations and phasing mutations. “This collaboration reflects our shared commitment to advancing genomic research through innovative, integrated solutions,” said Annemarie Watson, CEO of Covaris. “By combining our proven AFA-based extraction technology with PacBio’s sequencing expertise, we are enabling researchers to unlock valuable insights from even the most challenging FFPE samples, helping accelerate discoveries in cancer biology and beyond.”
